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Modification of Tannin extracted from the bark of Acacia auriculiformis for the antibacterial activity and application of metal adsorption

Authors:

S. Arasaretnam ,

Eastern University Sri Lanka, Chenkalady, LK
About S.
Department of Chemistry
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K. Venujah

Eastern University Sri Lanka, Chenkalady, LK
About K.
Department of Chemistry
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Abstract

Tannins offer capability as a bio-derived chemical feedstock. Their current application is limited primarily to leather browning and wood board adhesives. However, proper derivatization may change both the chemical and physical properties and thereby permit further utilization of polyphenols. This study is focus on tannin extraction and modification to obtain green polymeric materials. In this study, Tannin extracted from Acacia auriculiformis (Kaththi Karuvel) bark was modified by reacting with different aliphatic alkyl carboxylic acid (C4 and C18) to produce esterified tannin. Various weight percentage of alkyl chain lengths were introduced into the Tannin by esterification. The resulted modified Tannins were characterized by FT-IR and ATR spectroscopic techniques. Bivalent cations such as Mg2+,Cu2+ and Cd2+ were used to evaluate the adsorption properties of both the short and long chain fatty acid tannin ester. The esterified tannins were used to determine the inhibition effect against clinical isolates Staphylococcus aureus and Escherichia coli. Tests were conducted aerobically using nutrient agar medium. Vulnerabilities were determined using standard agar well-diffusion method. It was noticed that the esterified tannin showed effectual ion exchange and antibacterial ability compared to that of virgin tannin . It was found out that partially esterified products shown higher exchange capacity compared to fully esterified tannin. And also among this partially esterified products (C18-carboxylic acid ) long chain carboxylic acid ester products have shown highest value of ion adsorption compared to short chain (C4 carboxylic acid) acid-ester products. The resulting esters were used to study the ion exchange capacity of bivalent ions such as Cd2+, mg2+ and Cu2+.The highest ion adsorption capacity shown by the ester T1S1 for Cd2+ (0.020 mg/l), Mg2+ (13.852 mg/l) and Cu2+ (15.650 mg/l). In the case of antibacterial activity, the outcomes pointed out that these tannin esters possessed the highest antibacterial activity against the bacteria, Staphylococcus aureus at 2.5 mg/ml concentration(LD50-1.2). Amongst the gram-positive and gram-negative bacteria, gram-positive bacterial strains were more susceptible to the extracts when compared to gram negative bacteria.This was shown by the T1S6 Tannin esters.
How to Cite: Arasaretnam, S. & Venujah, K., (2017). Modification of Tannin extracted from the bark of Acacia auriculiformis for the antibacterial activity and application of metal adsorption. Ruhuna Journal of Science. 8(2), pp.90–102. DOI: http://doi.org/10.4038/rjs.v8i2.30
Published on 31 Dec 2017.
Peer Reviewed

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